Analysis of Changes in Malondialdehyde (MDA) and Lactate Dehydrogenase (LDH) Levels After a 30 km Cycling Event in The Makassar Cycling Community

Andi Eka Nur Wahyu(1*), Irfan Idris(2), Djohan Aras(3), Andi Wardihan Sinrang(4), Ilhamjaya Patellongi(5), Nukhrawi Nawir(6),

(1) Hasanuddin University
(2) Hasanuddin University
(3) Hasanuddin University
(4) Hasanuddin University
(5) Hasanuddin University
(6) Hasanuddin University
(*) Corresponding Author




DOI: https://doi.org/10.26858/cjpko.v15i1.43630

Abstract


This study looks at the changes in MDA and LDH levels after a 30 km cycling event in the Makassar cycling community. This research is a pre-experimental with a one-group pretest-posttest design on 30 male cyclists aged 30-60 years. The pre-test was carried out the day before the cycling event, and then the post-test was carried out 2 hours after the 30 km cycling event. Blood samples were tested using the ELISA Kit. Data were analyzed using the Wilcoxon test to see the effect of a 30 km cycling event on changes in MDA and LDH levels. Spearman correlation test to see the correlation between changes in MDA levels and LDH. The results of the study found that the 30 km cycling event had a significant effect on changes in MDA levels due to lipid peroxidation (p<0.05) and a significant effect on changes in LDH levels due to increased lactate secretion (p<0.05). There was no significant correlation between changes in MDA and LDH levels (p=0.60) with a negative correlation, which means that the more significant the change in MDA levels, the lower the LDH level.


Keywords


Cyclists; Malondialdehyde; Lactate Dehydrogenase; Oxidative Stress.

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References


Admin, Sari Octarina Piko, Rostika Flora, & Theodorus. (2019). Perbandingan Aktivitas Fisik Aerobik Dan Anaerobik Terhadap Kadar Laktat Dan Laktat Dehidrogenase (Ldh). Jurnal Kesehatan Dan Pembangunan, 9(17), 88–97. https://doi.org/10.52047/jkp.v9i17.33

Ayala, A., Muñoz, M. F., & Argüelles, S. (2014). Lipid peroxidation: Production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity, 2014. https://doi.org/10.1155/2014/360438

Bouchez, J. (2015). Pr ev iew - C op yr igh te d at er ial - C Pr ev yr igh te d.

Brancaccio, P., Lippi, G., & Maffulli, N. (2010). Biochemical markers of muscular damage. Clinical Chemistry and Laboratory Medicine, 48(6), 757–767. https://doi.org/10.1515/CCLM.2010.179

Chevion, S., Moran, D. S., Heled, Y., Shani, Y., Regev, G., Abbou, B., Berenshtein, E., Stadtman, E. R., & Epstein, Y. (2003). Plasma antioxidant status and cell injury after severe physical exercise. Proceedings of the National Academy of Sciences of the United States of America, 100(9), 5119–5123. https://doi.org/10.1073/pnas.0831097100

Fisher-Wellman, K., & Bloomer, R. J. (2009). Acute exercise and oxidative stress: A 30 year history. Dynamic Medicine, 8(1), 1–25. https://doi.org/10.1186/1476-5918-8-1

Flora, R. (2015). Pengaruh Latihan Fisik Anaerobik Terhadap Kadar Laktat Plasma dan Kadar Laktat Jaringan Otot Jantung Tikus Wistar Effect Anaerobic Exercise on The Blood Lactate Levels and Myocardium Levels in Wistar Rats. Journal Article, 1, 40–42.

Gea, J., Pascual, S., Casadevall, C., Orozco-Levi, M., & Barreiro, E. (2015). Muscle dysfunction in chronic obstructive pulmonary disease: Update on causes and biological findings. Journal of Thoracic Disease, 7(10), E418–E438. https://doi.org/10.3978/j.issn.2072-1439.2015.08.04

Goodwin, M. L., Harris, J. E., Hernández, A., & Gladden, L. B. (2007). Blood lactate measurements and analysis during exercise: A guide for clinicians. Journal of Diabetes Science and Technology, 1(4), 558–569. https://doi.org/10.1177/193229680700100414

Helianti, D., & Hairrudin. (2009). Efek Protektif Propolis Dalam Mencegah Stres Oksidatif Akibat Aktivitas Fisik Berat (Swimming Stress) “Propolis” Protective Effect to Prevent Oxidative Stress Caused by Strenous Physical Activity (Swimming Stress). Jurnal Ilmu Dasar, 10(2), 207–211.

Kawamura, T., & Muraoka, I. (2018). Exercise-induced oxidative stress and the effects of antioxidant intake from a physiological viewpoint. Antioxidants, 7(9). https://doi.org/10.3390/antiox7090119

Khonsary, S. (2017). Guyton and Hall: Textbook of Medical Physiology. In Surgical Neurology International (Vol. 8, Issue 1). https://doi.org/10.4103/sni.sni_327_17

Kim, H. J., Lee, Y. H., & Kim, C. K. (2007). Biomarkers of muscle and cartilage damage and inflammation during a 200 km run. European Journal of Applied Physiology, 99(4), 443–447. https://doi.org/10.1007/s00421-006-0362-y

Kobayashi, Y., Takeuchi, T., Hosoi, T., Yoshizaki, H., & Loeppky, J. A. (2005). Effect of a marathon run on serum lipoproteins, creatine kinase, and lactate dehydrogenase in recreational runners. Research Quarterly for Exercise and Sport, 76(4), 450–455. https://doi.org/10.1080/02701367.2005.10599318

Li, G., Lee, P., Mori, N., Yamamoto, I., & Arai, T. (2012). Long term intensive exercise training leads to a higher plasma malate/lactate dehydrogenase (M/L) ratio and increased level of lipid mobilization in horses. Veterinary Research Communications, 36(2), 149–155. https://doi.org/10.1007/s11259-012-9515-0

Lieberman, Marks, Allan D., Peet, Alisa., M. (2013). Marks’ basic medical biochemistry : a clinical approach. Wolters Kluwer Health/Lippincott Williams & Wilkins.

Michailidis, Y., Jamurtas, A. Z., Nikolaidis, M. G., Fatouros, I. G., Koutedakis, Y., Papassotiriou, I., & Kouretas, D. (2007). Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. Medicine and Science in Sports and Exercise, 39(7), 1107–1113. https://doi.org/10.1249/01.mss.0b013e318053e7ba

Oja, P., Titze, S., Bauman, A., de Geus, B., Krenn, P., Reger-Nash, B., & Kohlberger, T. (2011). Health benefits of cycling: A systematic review. Scandinavian Journal of Medicine and Science in Sports, 21(4), 496–509. https://doi.org/10.1111/j.1600-0838.2011.01299.x

Prasetyo, Y. (2015). Kesadaran Masyarakat Berolahraga Untuk Peningkatan Kesehatan Dan Pembangunan Nasional. Medikora, 11(2), 219–228. https://doi.org/10.21831/medikora.v11i2.2819

Rubio-Arias, J., Ávila-Gandía, V., López-Román, F. J., Soto-Méndez, F., Alcaraz, P. E., & Ramos-Campo, D. J. (2019). Muscle damage and inflammation biomarkers after two ultra-endurance mountain races of different distances: 54 km vs 111 km. Physiology and Behavior, 205, 51–57. https://doi.org/10.1016/j.physbeh.2018.10.002

Rupaka, A. P., Sulistyo, A. B., & Punia, D. (2021). Pemetaan Perilaku Pesepeda Pra dan Pasca Pandemi Covid-19 di Provinsi Bali Menggunakan Data Strava Metro. Jurnal Teknologi Transportasi Dan Logistik, 2(2), 119–126. https://doi.org/10.52920/jttl.v2i2.32

Saryono. (2011). METODOLOGI PENELITIAN KESEHATAN (A. Setiawan (ed.)). Mitra Cendikia Press.

Sinaga, F. A. (2016). Stress oksidatif dan status antioksidan pada aktivitas fisik maksimal. Jurnal Generasi Kampus, 9(2), 176–189.

Torres, S. H., Montes de Oca, M., Loeb, E., Zabner-Oziel, P., Wallis, V., & Hernández, N. (2009). Isoenzimas de lactatodeshidrogenasa en el músculo esquelético de pacientes con EPOC. Archivos de Bronconeumología, 45(2), 75–80. https://doi.org/10.1016/j.arbres.2008.04.001

Tyagita, N., Safitri, A. H., & Widayati, E. (2021). Penuaan dan stres oksidatif.

Vasudevan, D., S, S., & Vaidyanathan, K. (2016). Overview of Metabolism. In Textbook of Biochemistry for Medical Students. https://doi.org/10.5005/jp/books/13014_10

Völter, C., Götze, L., Dazert, S., Wirth, R., & Thomas, J. P. (2020). Impact of hearing loss on geriatric assessment. Clinical Interventions in Aging, 15, 2453–2467. https://doi.org/10.2147/CIA.S281627

Wahyuni, et al. (2021). Peningkatan Pengetahuan tentang Penerapan Frekuensi, Intensitas, Tipe, dan Waktu (FITT) dalam Olahraga Bersepeda pada Klub Gowes Puri Bolon Indah. Abdi Geomediains, 2(1), 51–60. https://journals2.ums.ac.id/index.php/abdigeomedisains/article/view/298/123...23 Okt 2021

Yunus, M. (2016). Pengaruh aktivitas badminton pada malam hari terhadap stres oksidatif (Studi Kasus Pada Mahasiswa Universitas Negeri Malang). Jurnal Kepelatihan Olahraga, 1(1), 1–5.


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