Expression of Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1α Increases After 8-Weeks of Endurance Training in Obese Females

Arya Dwi Prastickho(1), Desiana Merawati(2*), Sugiharto Sugiharto(3),

(1) State University of Malang
(2) State University of Malang
(3) State University of Malang
(*) Corresponding Author




DOI: https://doi.org/10.26858/cjpko.v15i2.47705

Abstract


This study aimed to prove the effect of moderate-intensity aerobic exercise on increasing Peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1α) levels in obese women. A total of 12 women aged 20-25, with body mass index (BMI) of 25-30 kg/m2, normal systolic blood pressure, and normal resting heart rate as subjects in the study. Subjects were randomly divided into 2 groups, namely K1 (n=6; control without intervention) and K2 (n=6; aerobic exercise). The exercise was carried out with an intensity of 60% -70% HRmax, frequency 3x/week for 8 weeks. Pretest and posttest PGC-1α levels were analyzed using the Enzyme-linked immunosorbent assay (ELISA) method. The data analysis technique uses the Paired Sample T-Test with a significant level of 5%. Based on the results of the study it was concluded that aerobic exercise with an intensity of 60% -70% HRmax with a frequency of 3x/week for 8 weeks affected increasing PGC-1α levels in obese women.


Keywords


Physical Exercise; PGC-1α levels; myokine; obesity; metabolism.

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References


Arhire, L. I., Mihalache, L., & Covasa, M. (2019). Irisin: A Hope in Understanding and Managing Obesity and Metabolic Syndrome. Frontiers in endocrinology, 10, 524. https://doi.org/10.3389/fendo.2019.00524.

Bae, J. Y., Woo, J., Kang, S., & Shin, K. O. (2018). Effects of detraining and retraining on muscle energy-sensing network and meteoric-like levels in obese mice. Lipids in health and disease, 17(1), 97. https://doi.org/10.1186/s12944-018-0751-3.

Balampanis, K., Chasapi, A., Kourea, E., Tanoglidi, A., Hatziagelaki, E., Lambadiari, V., et al. (2019). Inter-tissue expression patterns of the key metabolic biomarker PGC-1α in severely obese individuals: implication in obesity-induced disease. Hellenic Journal of Cardiology, 60(5), 282-293. https://doi.org/10.1016/j.hjc.2018.08.002.

Boström, P., Wu, J., Jedrychowski, M. P., Korde, A., Ye, L., Lo, J. C., Rasbach, K. A., Boström, E. A., Choi, J. H., Long, J. Z., Kajimura, S., Zingaretti, M. C., Vind, B. F., Tu, H., Cinti, S., Højlund, K., Gygi, S. P., & Spiegelman, B. M. (2012). A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature, 481(7382), 463–468. https://doi.org/10.1038/nature10777.

Castro, E. A., Carraça, E. V., Cupeiro, R., López-Plaza, B., Teixeira, P. J., González-Lamuño, D., & Peinado, A. B. (2020). The effects of the type of exercise and physical activity on eating behavior and body composition in overweight and obese subjects. Nutrients, 12(2), 557. https://doi.org/10.3390/nu12020557.

Collison, K. S., Zaidi, M. Z., Subhani, S. N., Al-Rubeaan, K., Shoukri, M., & Al-Mohanna, F. A. (2010). Sugar-sweetened carbonated beverage consumption correlates with BMI, waist circumference, and poor dietary choices in school children. BMC public health, 10, 234. https://doi.org/10.1186/1471-2458-10-234.

de Souza-Teixeira, F., Alonso-Molero, J., Ayán, C., Vilorio-Marques, L., Molina, A. J., González-Donquiles, C., Dávila-Batista, V., Fernández-Villa, T., de Paz, J. A., & Martín, V. (2018). PGC-1α as a Biomarker of Physical Activity-Protective Effect on Colorectal Cancer. Cancer prevention research (Philadelphia, Pa.), 11(9), 523–534. https://doi.org/10.1158/1940-6207.CAPR-17-0329.

Dinas, P. C., Lahart, I. M., Timmons, J. A., Svensson, P. A., Koutedakis, Y., Flouris, A. D., & Metsios, G. S. (2017). Effects of physical activity on the link between PGC-1a and FNDC5 in muscle, circulating Ιrisin and UCP1 of white adipocytes in humans: A systematic review. F1000Research, 6, 286. https://doi.org/10.12688/f1000research.11107.2.

Elizondo-Montemayor, L., Gonzalez-Gil, A. M., Tamez-Rivera, O., Toledo-Salinas, C., Peschard-Franco, M., Rodríguez-Gutiérrez, N. A., Silva-Platas, C., & Garcia-Rivas, G. (2019). Association between Irisin, hs-CRP, and Metabolic Status in Children and Adolescents with Type 2 Diabetes Mellitus. Mediators of inflammation, 2019, 6737318. https://doi.org/10.1155/2019/6737318.

Fang, P., He, B., Yu, M., Shi, M., Zhu, Y., Zhang, Z., & Bo, P. (2019). Treatment with celastrol protects against obesity through suppression of galanin-induced fat intake and activation of PGC-1α/GLUT4 axis-mediated glucose consumption. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1865(6), 1341-1350. https://doi.org/10.1016/j.bbadis.2019.02.002

Halling, J. F., & Pilegaard, H. (2020). PGC-1α-mediated regulation of mitochondrial function and physiological implications. Applied Physiology, Nutrition, and Metabolism, 45(9), 927-936. https://doi.org/10.1139/apnm-2020-0005.

Hejazi, K., Ziaaldini, M., Attarzadeh Hosseini, S. R., & Fathi, M. (2020). The impact of aerobic training intensity on skeletal muscle PGC-1α, interferon regulatory factor 4, and atherogenic index in obese male Wistar rats. Iranian Journal of Veterinary Science and Technology, 12(2), 50-58.

Ikeda, K., & Yamada, T. (2020). UCP1 dependent and independent thermogenesis in brown and beige adipocytes. Frontiers in endocrinology, 11, 498. https://doi.org/10.3389/fendo.2020.00498.

Jeong, H. J., Lee, H. J., Vuong, T. A., Choi, K. S., Choi, D., Koo, S. H., Cho, S. C., Cho, H., & Kang, J. S. (2016). Prmt7 Deficiency Causes Reduced Skeletal Muscle Oxidative Metabolism and Age-Related Obesity. Diabetes, 65(7), 1868–1882. https://doi.org/10.2337/db15-1500.

Knudsen, J. G., Murholm, M., Carey, A. L., Biensø, R. S., Basse, A. L., Allen, T. L., Hidalgo, J., Kingwell, B. A., Febbraio, M. A., Hansen, J. B., & Pilegaard, H. (2014). Role of IL-6 in exercise training- and cold-induced UCP1 expression in subcutaneous white adipose tissue. PloS one, 9(1), e84910. https://doi.org/10.1371/journal.pone.0084910.

Lanzi, S., Codecasa, F., Cornacchia, M., Maestrini, S., Salvadori, A., Brunani, A., & Malatesta, D. (2014). Fat oxidation, hormonal and plasma metabolite kinetics during a submaximal incremental test in lean and obese adults. PloS one, 9(2), e88707. https://doi.org/10.1371/journal.pone.0088707.

Laurens, C., Bergouignan, A., & Moro, C. (2020). Exercise-released myokines in the control of energy metabolism. Frontiers in physiology, 11, 91. https://doi.org/10.3389/fphys.2020.00091.

Lim, C., Kim, H. J., Morton, R. W., Harris, R., Phillips, S. M., Jeong, T. S., & Kim, C. K. (2019). Resistance Exercise-induced Changes in Muscle Phenotype Are Load Dependent. Medicine and science in sports and exercise, 51(12), 2578–2585. https://doi.org/10.1249/MSS.0000000000002088.

Liu, J., Lee, J., Salazar Hernandez, M. A., Mazitschek, R., & Ozcan, U. (2015). Treatment of obesity with celastrol. Cell, 161(5), 999–1011. https://doi.org/10.1016/j.cell.2015.05.011.

Lobstein, T., & Jackson-Leach, R. (2006). Estimated burden of paediatric obesity and co-morbidities in Europe. Part 2. Numbers of children with indicators of obesity-related disease. International journal of pediatric obesity : IJPO : an official journal of the International Association for the Study of Obesity, 1(1), 33–41. https://doi.org/10.1080/17477160600586689.

Lupita, M. N., Merawati, D., & Sugiharto, S. (2020). Secretion Of PGC-1α By Modulating Physical Exercise To Protect Improving Obesity Prevalence. STRADA Jurnal Ilmiah Kesehatan, 9(2), 345-352.

McKie, G. L., & Wright, D. C. (2020). Biochemical adaptations in white adipose tissue following aerobic exercise: from mitochondrial biogenesis to browning. The Biochemical journal, 477(6), 1061–1081. https://doi.org/10.1042/BCJ20190466.

Otero-Díaz, B., Rodríguez-Flores, M., Sánchez-Muñoz, V., Monraz-Preciado, F., Ordoñez-Ortega, S., Becerril-Elias, V., ... & Antuna-Puente, B. (2018). Exercise induces white adipose tissue browning across the weight spectrum in humans. Frontiers in physiology, 9, 1781. https://doi.org/10.3389/fphys.2018.01781.

Park, J. S., Holloszy, J. O., Kim, K., & Koh, J. H. (2020). Exercise training-induced PPARβ increases PGC-1α protein stability and improves insulin-induced glucose uptake in rodent muscles. Nutrients, 12(3), 652. https://doi.org/10.3390/nu12030652.

Pekkala, S., Wiklund, P. K., Hulmi, J. J., Ahtiainen, J. P., Horttanainen, M., Pöllänen, E., Mäkelä, K. A., Kainulainen, H., Häkkinen, K., Nyman, K., Alén, M., Herzig, K. H., & Cheng, S. (2013). Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health?. The Journal of physiology, 591(21), 5393–5400. https://doi.org/10.1113/jphysiol.2013.263707.

Petridou, A., Siopi, A., & Mougios, V. (2019). Exercise in the management of obesity. Metabolism: clinical and experimental, 92, 163–169. https://doi.org/10.1016/j.metabol.2018.10.009.

Pranoto, A., Cahyono, M. B. A., Yakobus, R., Izzatunnisa, N., Ramadhan, R. N., Rejeki, P. S., Miftahussurur, M., Effendi, W. I., Wungu, C. D. K., & Yamaoka, Y. (2023). Long-Term Resistance-Endurance Combined Training Reduces Pro-Inflammatory Cytokines in Young Adult Females with Obesity. Sports (Basel, Switzerland), 11(3), 54. https://doi.org/10.3390/sports11030054.

Pranoto, A., Rejeki, P. S., Miftahussurur, M., Setiawan, H. K., Yosika, G. F., Munir, M., Maesaroh, S., Purwoto, S. P., Waritsu, C., & Yamaoka, Y. (2023). Single 30 min treadmill exercise session suppresses the production of pro-inflammatory cytokines and oxidative stress in obese female adolescents. Journal of basic and clinical physiology and pharmacology, 34(2), 235–242. https://doi.org/10.1515/jbcpp-2022-0196.

Rejeki, P. S., Pranoto, A., Rahmanto, I., Izzatunnisa, N., Yosika, G. F., Hernaningsih, Y., Wungu, C. D. K., & Halim, S. (2023). The Positive Effect of Four-Week Combined Aerobic-Resistance Training on Body Composition and Adipokine Levels in Obese Females. Sports (Basel, Switzerland), 11(4), 90. https://doi.org/10.3390/sports11040090.

Shirvani, H., & Arabzadeh, E. (2020). Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α. Eating and Weight Disorders- Studies on Anorexia, Bulimia and Obesity, 25(1), 17-24. https://doi.org/10.1007/s40519-018-0491-4.

Skovgaard, C., Brandt, N., Pilegaard, H., & Bangsbo, J. (2016). Combined speed endurance and endurance exercise amplify the exercise‐induced PGC‐1α and PDK4 mRNA response in trained human muscle. Physiological reports, 4(14), e12864. https://doi.org/10.14814/phy2.12864.

Sugiharto, Merawati, D., Pranoto, A., Rejeki, P.S., Lupita, M.N., Adji, B.S., Susanto, H., & Taufiq, A. (2021). Acute Interval and Continuous Moderate-Intensity Exercise Enhanced Circadian Thermogenic Activity through Browning-related Genes in Obese Adolescent Female. Malaysian Journal of Fundamental and Applied Sciences, 17(5), 566-581. https://doi.org/10.11113/mjfas.v17n5.2271.

Vaccari, F., Passaro, A., D'Amuri, A., Sanz, J. M., Di Vece, F., Capatti, E., Magnesa, B., Comelli, M., Mavelli, I., Grassi, B., Fiori, F., Bravo, G., Avancini, A., Parpinel, M., & Lazzer, S. (2020). Effects of 3-month high-intensity interval training vs. moderate endurance training and 4-month follow-up on fat metabolism, cardiorespiratory function and mitochondrial respiration in obese adults. European journal of applied physiology, 120(8), 1787–1803. https://doi.org/10.1007/s00421-020-04409-2.

Yang, S., Loro, E., Wada, S., Kim, B., Tseng, W. J., Li, K., Khurana, T. S., & Arany, Z. (2020). Functional effects of muscle PGC-1alpha in aged animals. Skeletal muscle, 10(1), 14. https://doi.org/10.1186/s13395-020-00231-8.


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