Variasi Genetik (Polimorfisme) Anopheles barbirostris Dengan Menggunakan Metode PCR-RAP
(1) Balai Penelitian dan Pengembangan Kesehatan, Donggal
(2) Balai Penelitian dan Pengembangan Kesehatan, Donggal
(3) Balai Penelitian dan Pengembangan Kesehatan, Donggal
(4) Badan Pengkajian dan Penerapan Teknologi, Jakar
(*) Corresponding Author
Abstract
ABSTRACT
Malaria is still a public health problem in Indonesia. Several Anopheles mosquitoes have been identified as malaria vector in in various both of inland and mountain ecosystems. The aim of this study was to analyze the genetic variation (polymorphism) among Anopheles barbirostris in inland and mountain ecosystems in Sigi regency. The level of genetic polymorphism was obtained by Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) method. Analysis was conducted by comparing the DNA bands patterns to identify polymorphic and monomorphic among this species. The results showed that 57% of An. barbirostris from inland ecosystems were polymorphism and others of 43% were monomorphism. In the mountain ecosystems, about 53% of An. barbirostris were polymorphisms and 47% were monomorphism. in general An. barbirostris in inland ecosystems and in mountainous ecosystems tend to have genetic similarities.
Keywords : Anopheles barbirostris, polymorphism, inland ecosystem, mountain ecosystem
Malaria is still a public health problem in Indonesia. Several Anopheles mosquitoes have been identified as malaria vector in in various both of inland and mountain ecosystems. The aim of this study was to analyze the genetic variation (polymorphism) among Anopheles barbirostris in inland and mountain ecosystems in Sigi regency. The level of genetic polymorphism was obtained by Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) method. Analysis was conducted by comparing the DNA bands patterns to identify polymorphic and monomorphic among this species. The results showed that 57% of An. barbirostris from inland ecosystems were polymorphism and others of 43% were monomorphism. In the mountain ecosystems, about 53% of An. barbirostris were polymorphisms and 47% were monomorphism. in general An. barbirostris in inland ecosystems and in mountainous ecosystems tend to have genetic similarities.
Keywords : Anopheles barbirostris, polymorphism, inland ecosystem, mountain ecosystem
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