Isolasi Dan Identifikasi Senyawa Metabolit Sekunder Ekstrak n-Heksana Tumbuhan Cakar Ayam (Selaginella plana Hieron)
(1) Jurusan Kimia FMIPA UNM Makassar
(2) Jurusan Kimia FMIPA UNM Makassar
(3) Jurusan Kimia FMIPA UNM Makassar
(*) Corresponding Author
DOI: https://doi.org/10.35580/chemica.v19i2.12775
Abstract
Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa metabolit sekunder yang terdapat dalam ekstrak n-heksana tumbuhan cakar ayam (Selaginella plana Hieron). Penelitian dilakukan melalui beberapa tahap yaitu preparasi sampel, maserasi dengan metanol, partisi dengan n-heksana, fraksinasi, pemurnian, dan identifikasi. Isolat diperoleh dalam bentuk kristal jarum berwarna putih dengan titik leleh 128 – 1300C dan memberikan hasil positif pada pereaksi Lieberman-Buchard. Dan spektrum IR isolat menunjukkan adanya gugus -OH (3425,58 cm-1), CH3- dan -CH2- alifatik (2958,80 cm-1; 2929,87 cm-1; 2895,15 cm-1; dan 2866,22 cm-1), C=C alkena (1641,42 cm-1), CH3- dan -CH2- tekuk (1462,04 cm-1 dan 1377,17 cm-1), C-O (1058,92 cm-1), =C-H (962,48 cm-1). Berdasarkan data tersebut disimpulkan bahwa isolat yang diperoleh merupakan golongan steroid.
Kata kunci : Isolasi, Selaginella plana Hieron, Metabolit Sekunder, Steroid
ABSTRACT
The purpose of this research was to isolate and identifed the secondary metabolite compound from n-hexane extract of Cakar Ayam (Selaginella plana Hieron) plants. This research was carried out in several steps, they were preparation of sample, maceration with methanol, partition with n-hexane, fractination, purification, and identification. The isolate obtaned was white crystal needle shaped with melting point to 128-1300C and gave positive respon to the Liebermann-Buchard test. The IR spectrum of the isolate showed the presence of -OH (3425,58 cm-1),CH3- and -CH2- alifatic (2958,80 cm-1; 2929,87 cm-1; 2895,15 cm-1; and 2866,22 cm-1),C=C alkena (1641,42 cm-1),CH3- and -CH2- bending (1462,04 cm-1 and 1377,17 cm-1), C-O (1058,92 cm-1),=C-H (962,48 cm-1). Based on those result, it was concluded that the isolate was a steroid compound.
Keywords : Isolation, Selaginella plana Hieron, Secondary Metabolites, Steroid
References
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ISSN: 2722-8649
Diterbitkan oleh Jurusan Kimia FMIPA Universitas Negeri Makassar (UNM) dua kali dalam setahun yaitu bulan Juni dan Desember.
Alamat Redaksi :Jurusan Kimia, Fakultas MIPA UNM, JL. Dg. Tata Parangtambung, Makassar 90224 Indonesia, Telp. 0411-840295; Fax: 0411840295;E-mail : chemica@unm.ac.id