Pengaruh Penggunaan Sumber Silika Berbeda Terhadap Pertumbuhan dan Produktivitas Biomassa Diatom Skeletonema sp. (Bacillariophyceae)

Indrayani Indrayani(1*), Haslianti Haslianti(2), Asmariani Asmariani(3), Ardiansyah Ardiansyah(4),

(1) Universitas Negeri Makassar
(2) Universitas Halu Oleo
(3) Universitas Halu Oleo
(4) Politeknik Pertanian Negeri Pangkep
(*) Corresponding Author




DOI: https://doi.org/10.26858/jptp.v8i2.35430

Abstract


Pertumbuhan dan produktivitas biomassa mikroalga khususnya diatom (Bacillariophyceae) sangat dipengaruhi oleh keberadaan silika sebagai penyusun dinding sel (frustule).   Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan silika yang berbeda terhadap pertumbuhan dan produktivitas biomassa mikrolga laut Skeletonema sp. Mikroalga Skeletonema sp. dikultur menggunakan media F/2 dengan sumber silikat yang berbeda yakni Sodium Metasilicate  Powder (Merck), Sodium silicate solution PA (Merck) dan waterglass silikat Teknis pada salinitas 32 ppt, suhu kamar, siklus gelap dan terang 12jam:12 jam selama dua minggu dengan batch mode (in triplicates).  Hasil penelitian menunjukan bahwa Skeletonema sp. tumbuh baik pada semua jenis silika yang digunakan.   Terdapat perbedaan yang signifikan antara laju pertumbuhan spesifik  (LPS) waterglass silika dengan silika solution  (P=0.018) serta antara Sodium silika powder dengan silika solution (P=0.024) namun  tidak terdapat perbedaan yang signifikan antara waterglass silika dan silika powder  (P=0.560).  LPS tertinggi diperoleh pada penggunaan waterglass silika (0,405±0,043d-1) dan terendah pada penggunaan silika solution (0,290±0,037d-1).  Biomass yield antara masing-masing media kultur tidak menunjukkan perbedaan yang signifikan (P=0,770).  Biomass yield berkisar antara 0,60-0,62 g.L-1.  Terdapat perbedaan yang signifikan pada produktivitas biomass antara masing-masing perlakuan (P=0.023).  Produktivitas biomassa tertinggi diperoleh pada penggunaan waterglass silika (0,252±0,033 g.L-1.d-1) dan terendah  pada penggunaan silika solution (0,232±0,015g.L-1.d-1).  Penelitian ini menunjukkan bahwa penggunaan waterglass silika memberikan  pertumbuhan dan produktivitas biomassa tertinggi.  Selain itu waterglass silika jauh lebih murah sehingga lebih ekonomis

Keywords


Diatom; Skeletonema sp.; silika; pertumbuhan

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References


Andersen, R.A., Kawachi, M. (2005). Traditional microalgae isolation techniques. In: Anderson RA (eds) Algal Culturing Techniques. Elsevier. Amsterdam.

Ebrahimi, E.; Salarzadeh, A. (2016). The Effect of Temperature and Salinity on the Growth of Skeletonema costatum and Chlorella capsulate in Vitro. Int. J. Life Sci. 6 (10), 40–44

Egge, J.K., Aksnes, D.L. (1992). Silicate as regulating nutrient in phytoplankton competition. Mar. Ecol. Prog. Ser. 83:281-289

Fitrah, Indrayani, Emiyarti. (2022). Produksi Lipid Mikroalga Laut Skeletonema sp. Hasil Kultur Massal di Outdoor Raceway Pond Sebagai Bahan Baku Biodiesel. Jurnal Sains dan Inovasi Perikanan 6(1):10-18.

Guillard, R.R., Ryther, J.H. (1962). Studies of marine planktonic diatoms. 1. Cyclotella chui Hustedt, and Detonula confervacea (Cleve). Can J Microbiol 8: 229-239.

Hildebrand, M., Dahlin, K., Volcani, B.E. (1998). Characterization of a silicon transporter gene family in Cylindrotheca fusiformis: sequences, expression analysis, and identification of homologs in other diatoms. Mol Gen Genet 260:480-486

Hildebrand, M., Volcani, B.E., Gassmann, W., Schroeder, J.J. (1997). A gene family of silicon transporters. Nature 385:68-89

Indrayani, I., Haslianti, Asriyana. (2018). Isolation and screening of marine microalgae from Kendari waters, Southeast Sulawesi, Indonesia suitable for outdoor mass cultivation in hypersaline media. AACL Bioflux 11 (5): 1445-1455.

Indrayani, I. (2017). Isolation and characterization of microalgae with commercial potential. PhD thesis, Murdoch University, Perth, Australia, 214 pp

.Indrayani, I., Moheimani, N.R., Borowitzka, M.A. (2019). Long-term reliable culture of a halophilic diatom, Amphora sp.MUR258, in outdoor raceway ponds. Journal of Applied Phycology 31:2771–2778 https://doi.org/10.1007/s10811-019-01803-y

Indrayani, I., N.R. Moheimani., K. de Boer., P.A. Bahri., M.A. Borowitzka. (2020). Temperature and salinity effects on growth and fatty acid composition of a halophilic diatom, Amphora sp. MUR258 (Bacillariophyceae). Journal of Applied Phycology 32:977–987. Doi 10.1007/s10811-020-02053-z.

Indrayani, I., Haslianti, H., Asmariani, A., Muskita, W.H., Balubi, M. (2020). Growth, biomass and lipid productivityof a newly isolated tropical marine diatom, Skeletonemasp.UHO29, under different light intensities. Biodiversitas 21 (4): 1498-1503. DOI: 10.13057/biodiv/d210430

Jiang, Y., Katherine, S.L., Jola, B., Lou, B., Jennifer, C., Mark , B., Antonietta , Q. (2015). Effect of silicate limitation on growth, cell composition, and lipid production of three native diatoms to Southwest Texas desert. Journal of Applied Phycology 27:1433–1442. DOI 10.1007/s10811-014-0463-7

Kaeriyama,H., Katsuki, E., Otsubo, M., Yamada, M., Ichimi, K., Tada, K., Harrison, P.J. (2011). Effects of Temperature and Irradiance on Growth of Strains Belonging to Seven Skeletonema Species Isolated from Dokai Bay, Southern Japan. Eur. J. Phycol., 46(2): 113–124

Kemp, A.E.S., Pike, J., Pearce, R.B., C.B. Lange, C.B., (2000), ‘The “Fall dump” – a new perspective on the role of a “shade flora” in the annual cycle of diatom production and export flux‘. Deep-Sea Res. II 47: 2129–2154.

Lebeau, T., Bagot, D., Jezequel, K., Fabre, B. (2002). ‘Cadmium Biosorption by Free and Immobilised Micro-organisms Cultivated in a Liquid Solid Extract Medium: Effect of Cd, pH and Technique of Culture’. Sci. Tot. Environ. 291:73-83.

Lebeau, T., Robert, J.M. (2003). ‘Diatom Cultivation and Biotechnologically Relevant Products. Part II: Current and Putative Products’. Applied Microbiology Biotechnology 60: 624-632.

Moheimani, N.R., Borowitzka, M.A., Isdepsky, A., Fon Sing, S. (2013). Standard methods for measuring growth of algae and their composition. In: Borowitzka MA, Moheimani NR (eds.) Algae for Biofuels and Energy. Springer, Dordrecht.

Papush, L., Danielsson, A. (2006). Silicon in the marine environment: Dissolved silica trends in the Baltic Sea. Estuarine, Coastal and Shelf Science 67:53-66.

Reynolds, C. (2006). Ecology of phytoplankton. Cambridge University Press, Cambridge.

Round, F., Crawford, R., Mann, D. (1990). The Diatom, Cambridge University Press, Cambridge, UK.

Stoermer, E.F., Smol, J.P. (2001). The Diatoms: Applications for the Environmental and Earth Sciences, Cambridge University Press, Cambridge, UK.

Steinrücken, P., Mjøs, S.A., Prestegard, S.K., Erga, S.R. (2018). Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors. Front. Plant Sci. 9:491. doi: 10.3389/fpls.2018.00491

Taraldsvik, M., Myklestad, S.M. (2000). The effect of pH on growth rate, biochemical composition and extracellular carbohydrate production of the marine diatom Skeletonema costatum. Eur. J. Phycol.35:189–194.


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