THE EFFECT OF SUPERCRITICAL CO2 ON THE ANTIOXIDANT ACTIVITY OF THE SWIETENIA MAHAGONI SEED EXTRACT BY BOX-BEHNKEN DESIGN

Hartati Hartati(1*), Liza Md Salleh(2), Mohd. Azizi Che Yunus(3),

(1) 
(2) 
(3) 
(*) Corresponding Author

Abstract


In this study, the influence of supercritical carbon dioxide parameter on the antioxidant activity on Swietenia mahagoni seed extract was investigated. Response surface methodology was employed, using Box-Behnken statistical design to evaluate the effects of three independent variables. Pressure (20-30 MPa), temperature (40-60 oC) and particle size (0.25-0.75 mm) were the three variables with total time and CO2 flow rate being held constant at 180 minutes and 2 ml/min respectively, for each extraction. The extracts were screened for possible antioxidant activitiy by free radical scavenging activity 2,2-diphenyl-1-picryl hydrazyl (DPPH) assays. The study reveal that the highest antioxidant activity which was 94.84% was obtained at temperature of 40oC, pressure of 25 MPa and particle size of 0.25 mm. They stated that the dominan effect on the antioxidant activity of the Swietenia mahagoni seed extracts was the pressure campared to temperature and particle size.

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Copyright (c) 2017 Hartati Hartati, Liza Md Salleh, Mohd. Azizi Che Yunus

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