Isolasi dan Karakterisasi Enzim Bromelain dari Bonggol dan Daging Buah Nanas (Ananas comosus)
(1) Jurusan Kimia FMIPA UNM Makassar
(*) Corresponding Author
DOI: https://doi.org/10.35580/chemica.v21i2.17983
Abstract
Kata kunci: Ananas comosus, bonggol nanas, bromelain, aktivitas spesifik, pemurnian
ABSTRACT
The aim of this study was to isolate and purify the bromelain extracted from part of pineapple fruit (Ananas comosus) through fractionation method using ammonium sulfate followed by dialysis. The highest specific activity on ammonium sulfate fraction was 50-80% (fraction 3) both for the sample of the core and the flesh of pineapple, each was 0.30 U/mg and 0.21 U/mg. The fraction 3 of the core had a purity level 132.65 times of the crude enzyme while fraction 3 of the pineapple flesh had a purity level 108.47 times of the crude enzyme. From the dialysis process found the highest value of specific activity on fraction 3 of the pineapple core of 0.33 U/mg with a purity level of and 141.58 times of the crude enzyme. Fraction of the core has higher thermal stability than the fraction of the flesh. The optimum temperature and pH of this enzyme was 37oC and 7.0.
Keywords: Ananas comosus, pineapple core, bromelain, specific activity, purification
Full Text:
PDFReferences
Bhattacharyya, B.K. 2008. Bromelain. India: Biotechnology and Molecular Biology, East India Pharmaceutical Works Ltd. Vol 7(4).
Bresolin, et al. 2013. Isolation and purification of bromelain from waste peel of pineapple for therapeutic application. Brazil: Campinas, SP.
Gautam, S.S., et al. 2010. Comparative Study of Extraction, Purification, and Estimation of Bromelain from Stem and Fruit of
Pineapple Plant. India: Department of Pharmaceutics.
Lusianawati. 2013. Aktivitas Ekstrak dan Nanopartikel Ekstrak Kulit Kayu Mahoni sebagai Inhibitor EnzimHmg-Koa Reduktase. Bogor: Departemen Biokimia FMIPA Institut Pertanian Bogor.
Maulina, E.P. 2015. Studi Awal Uji Toksisitas Ekstrak Daun dan Batang Beligo (Benincasa Hispida) sebagai Inhibitor Α-Glukosidase. Depok: Departemen Kimia FMIPA Universitas Indonesia.
Maurer, H.R., 2001. Bromelain: biochemistry,pharmacology and medical use. Germany: Department of Biochemistry, Molecular Biology and Biotechnology, Institute of Pharmacy, University of Berlin.
Neta, J.L.V., et al. 2012. Bromelain Enzyme from Pineapple: In-vitro Activity Study under Different Micropropagation Conditions. Brazil: Springer Science.
Ramalingam, C., Srinath, R., Islam, N.N.2012. Isolation and Characterization of Bromelain from Pineapple (Ananas Comosus) and Comparing its anti-browning activity on apple juice with anti-browning agents. India: Elixir Food Science.
Rugayah, Anggalia, I., Cahya, Y. 2012. Pengaruh Konsentrasi dan Cara Aplikasi Iba (Indole Butiric Acid) terhadap Pertumbuhan Bibit Nanas (Ananas Comosus [L.] Merr.) Asal Tunas Mahkota. Bandar Lampung: Jurusan Agroteknologi Fakultas Pertanian Universitas Lampung.
Article Metrics
Abstract view : 3653 times | PDF view : 1139 timesRefbacks
- There are currently no refbacks.
ISSN: 2722-8649
Diterbitkan oleh Jurusan Kimia FMIPA Universitas Negeri Makassar (UNM) dua kali dalam setahun yaitu bulan Juni dan Desember.
Alamat Redaksi :Jurusan Kimia, Fakultas MIPA UNM, JL. Dg. Tata Parangtambung, Makassar 90224 Indonesia, Telp. 0411-840295; Fax: 0411840295;E-mail : chemica@unm.ac.id