A Simplified Model of Aspergillus niger Growth using Nephrolepis biserrata leaves for Exo-polygalacturonase Production

Abstract

A simple model to describe the growth of Aspergillus niger during solid state fermentation using Nephrolepis biserrata leaves is a substrate for the production of exo-poligalacturonase for 192 hours. The rate of kinetics growth of Aspergillus niger and the use of glucose as substrate using a Model Monod has shown that the maximum utilization of glucose 0.32 g/L of Aspergillus niger biomass of 0.496 mg/gdsf of glucose uptake 5.96 g/L to 0.32 g/L at 24 hours up to 120 hours of incubation. Through a linear regression equation diperolehinilai μ_max = 0.043 per hour and Ks = 0.473 g/L for 120 hours of incubation. The values of kinetic parameters and the amount of biomass has a significant relationship with R² equal to 0.801 and exo-polygalacturonase optimum activity at 54.64 U/g. Data gathered for the kinetic parameters during evaluation of the production of exo-polygalacturonase in SSF by Aspergillus niger, a value Y_X/S is 0.149 mg/g by using glucose from the medium. The values for the kinetic coefficient of exo-polygalacturonase income is Y_E/S = 16.43 U / g, Y_E/X = 114.4 U/mg and kinetic constants were qE = 4.79 U/(mg.h) and qS = 0.289 g/(mg.h).